LOCUS       sequence-55197-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBLC
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..123)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(124..141)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(142..162)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc ggatcctcac ttatcgtcgt catccttgta
      121 atcatggtgg tgatgatggt gcgattggaa gtagaggttc tctgcgccga gctcagtcag
      181 gtctgggttg tgggtctttc catctgggta gaggtagaag ccgtccttct gtccctccag
      241 gagcacctgg gacaggggtt tgttggcagg gatggtctgc aggatgctgc catctgagct
      301 cacatagccg atggcccact gccccaggcg agtacagctg ggccggaaga tgtaactgcc
      361 tggcttgtcc ctgcaggcct gcagacgctc ttggacctca tcataggtga ggaaggccat
      421 gtagcctggg tggttgactg ccaggagctg ccagttcttg aggagtgttg gccatggctg
      481 aaagagcctg gtgaagacgt cgaactcgaa gatggacacg tgcccgctgc aggtgaggtc
      541 aatggtggtg cgcaaggcca gggctgtgca gcctggttcc acagggtggc aggtgcccag
      601 gagggactca aactcagccc agggcagcac acaccgggct ccgcaacttt ccctccagaa
      661 ggtgtgggcg ggggccttgg tgagctggta catgtgtcca cagtactttc ccccggggaa
      721 gagtgcgtgc agctctgcgt gcatgtggct gaagatgatg gccagcttgg ccagctgtcg
      781 cctgagtctg gagcccgccc ggaagagctc gtcgttggca ctccttcggc cccggggagg
      841 cagcagcgcg gccacctgcc tgctcttggc ctccagattg gccaggtaga tgagtagaaa
      901 gtccccagag ccgccgggac ccccggggcc gcctccgccg gcctcccgcc gagaatgggc
      961 cacctctcga agcagctgcg ctgtgcgggg cagcaggtcc cgcagcgaag gga
//
