LOCUS       sequence-55200-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72110-BBa_J72103
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     157..174
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttattaatca gataaaatat ttctagaggc ctcccctgat tctgtggata accgggatct
       61 gtaaggatca accactttgt acaagaaagc tgggtcgaat tgattaccgc ctttgagtga
      121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc
      181 tgcacgtcga gttaggatcc gaacggttta ttacgtacat caggtaaaac tgaccgataa
      241 gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag
      301 taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccga taagccgctt
      361 tcttttgggt atagtgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac
      421 ccgccgaagc gggtttttac gtaaaccagg tgaaactgac cgataagccg ctttcttttg
      481 ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga
      541 agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggttctg tcgtggacag
      601 tcattcatct aggccagcaa tcgccagatc cttaatcatc atccccgtac agtttgcggg
      661 taacttcttc gtcttcctcc tcgtcttcgt ccagatcctg ttcaagccat gcttcgtttg
      721 atatgccttc cgcatcgaca tcaccataac cacctgtatc gacgatatcg gcaatttctt
      781 ctctacgatg ctcaatccac aatgcggcat cagcgcggcg gctggcggcc cgttctcgcg
      841 tgattttatc cagatcctca agtgatggag cgccagatgc tgtttggttt
//
