LOCUS       sequence-55214-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insect expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     Unpublished plasmid
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMT-puro
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    179..313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(216..235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     270..289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     315..332
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(355..372)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(382..423)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(520..946)
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
ORIGIN
        1 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
       61 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga
      121 agccacccgg ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagaa
      181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      301 tcatgtctgg atcttagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
      361 atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt
      421 accttcgaag ggccctctag actcgagcgg ccgccactgt gctggatatc tgcagaattc
      481 caccacactg gactagtagg taccccgatc tagatccccc ctttagttgc actgagatga
      541 ttcacttgat tttgctgctg accacaactg atgcacattg gccagatgct ctcggtttta
      601 tacattttgg aaacgcggct ttacacacgg gtctttcgtt cgattcacct cttttgcaca
      661 cgccggccgg ctccaaattc gagtggagac gtgtgcagaa gcttttgttc tgcccgcgaa
      721 gaattggttc ggttctccac tttggggact tgtatgggat gcaaagctct tggtggggca
      781 catggggcat gcgcaggcac tcttacttgc gtaccacata tgtatggggg tggcggtggg
      841 cggtgggggg ccggagttct gggtctctta tcggaaccag aggataggac ggcctgcagc
      901 aaagagctag tcacatcggg caccacatcc tgtcctgcaa cgaattaat
//
