LOCUS       sequence-55218-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insect expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     Unpublished plasmid
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMT-puro
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..81
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(131..148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    150..284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(174..193)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     228..247
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(489..509)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcacagtggc ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct
       61 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
      121 cgctgatcag cctcgactgt gccttctaag atccagacat gataagatac attgatgagt
      181 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      241 ctattgcttt atttgtaacc attataagct gcaataaaca agttctagag tcggtgggcc
      301 tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg cttcggtcgg agccatgggg
      361 tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt
      421 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
      481 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
      541 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
      601 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
      661 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
      721 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
      781 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
      841 cgtga
//
