LOCUS       sequence-55230-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72214-BBa_J72090
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      38..132
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             731..739
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
       61 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
      121 attggtgaga atccaagcct cggatcctta gcgcttggcc gcgaaaaccg cgtccgggat
      181 ctccgcctcg ctcacattcg gaaaatcacg caggcttgca cgcgacgctt catcgccggt
      241 gaccatgcgc agtggagtgc cgctcgccgc atccaggtac agggtgctcg gacctttacc
      301 tgggcgctgg taacgccaac cgtccgcttc gcgacccaga accacgcgac ggccaatgtg
      361 acgggcaccc agacggcgca ggacatcgcg tggcaggaac aattcggcaa aagggccagt
      421 ctcatcatcc agcggacgct cacacagctg ttcacccagg gcggtacgct cacgaccata
      481 ggcgactttt tgtttccagg tacggccgct ggccgcatta ccaacctccg acatccacag
      541 gcgataacca gtatcacgct cgctccacgg attgaacagg ccgtcaatac gacagatgtc
      601 acgttcatag tcgaaccagc aatagccgct ggtcagctgg tcatccggca gcatcgggct
      661 ccaataagag acataggcgc tgctccaacg ggctggcaac agtggtggct cacggttctc
      721 catttttttt acctccttaa agatcatcaa ttcacgcgac caatcttgta ccaaacattc
      781 tgtttacgct ggatttgttg cagacgatcg tacggctcgc tcgtaaagta cagtttctcg
//
