LOCUS       sequence-55271-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Doench JG, Sharp PA
  TITLE     Specificity of microRNA target selection in translational 
            repression.
  JOURNAL   Genes Dev. 2004 Mar 1. 18(5):504-11.
  PUBMED    15014042
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Mar 1. 18(5):504-11."
COMMENT     Sequence Label: pRL-TK 4x mut I
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    255..376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(266..285)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     320..339
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        509..613
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(832..851)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagaaagt
      121 tttcacaaag ctaacaggta ccaagttttc acaaagctaa gtccggaagt tttcacaaag
      181 ctaagttgta ccaagttttc acaaagctaa caccggatcg cgggccctag agcggccgct
      241 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
      301 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      361 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
      421 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
      481 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      541 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      601 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      661 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      721 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      781 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      841 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      901 tcagaatgac ttggttgagt actcaccagt cacag
//
