LOCUS       sequence-55283-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Muzumdar MD, Tasic B, Miyamichi K, Li L, Luo L
  TITLE     A global double-fluorescent Cre reporter mouse.
  JOURNAL   Genesis. 2007 Sep . 45(9):593-605.
  PUBMED    17868096
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Sep . 45(9):593-605."
COMMENT     Sequence Label: Rosa26 mT/mG
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..58)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(215..217,218..730)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(257..278)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(518..537)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgggctgca ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat
       61 taagggttcc ggatcatcac cgcggatggg ttgctgtttc tccaagaccg cagcgaaggg
      121 agaagccgcc gcggagaggc ctggggaggc ggctgtggcc tcgtcgcctt ccaaagcgaa
      181 cggacaggag aatggccacg tgaaggtagg atccgtgagc aagggcgagg agctgttcac
      241 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      301 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      361 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      421 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      481 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      541 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      601 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      661 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      721 caacatcgag gacggcagcg tgcagctcgc cgaccacta
//
