LOCUS       sequence-55288-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M38 TOP-dGFP
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(165..184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(166..184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    189..323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(213..232)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     267..286
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(436..456)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(436..454)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(475..491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(488..510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(523..529,530..547,548..553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    568..589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(706..723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caagaagctt agccatggct tcccgccgga ggtggaggag caggatgatg gcacgctgcc
       61 catgtcttgt gcccaggaga gcgggatgga ccgtcaccct gcagcctgtg cttctgctag
      121 gatcaatgtg taggcggccc ctctcgagcc tctagattct gcagccctat agtgagtcgt
      181 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      241 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      301 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      361 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
      421 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag
      541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaa
//
