LOCUS       sequence-55290-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M38 TOP-dGFP
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(95..113)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(96..113)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    311..445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(335..354)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     389..408
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(638..657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccatggtggc gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaatcg
       61 atgggatcct gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt
      121 agttgctcgc tgcagtcgcg gttggagtag taggcgcctc agctggcgcc tttatagaag
      181 cgctgtgaat ggatggactt cctacgtcac tggggtcgac tgaagctcct cctttgatct
      241 tacccccttt gatcttaccc cctttgatct tacccccttt gatagcttgg gctgcaggtc
      301 gactctagag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      361 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      421 cattataagc tgcaataaac aagttaacaa caattgcatt ccatttatgt ttcactcgac
      481 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      541 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      781 cg
//
