LOCUS       sequence-55304-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Smyth JW, Vogan JM, Buch PJ, Zhang SS, Fong TS, Hong TT, Shaw RM
  TITLE     Actin cytoskeleton rest stops regulate anterograde traffic of 
            connexin 43 vesicles to the plasma membrane.
  JOURNAL   Circ Res. 2012 Mar 30;110(7):978-89. Epub 2012 Feb 9.
  PUBMED    22328533
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res.";
            date: "2012-03-30"; volume: "110(7)"; pages: "978-89. Epub 2012 Feb 
            9"
COMMENT     Sequence Label: pDEST/LIfeAct-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             92..142
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     protein_bind    complement(156..180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(345..363)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     613..632
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcatcaaaca agtttgtaca aaaaagcagg
       61 ctttaaagga accaattcag tcgactggat catgggcgtg gccgacctga tcaagaagtt
      121 cgagagcatc agcaaggagg agtcgagata tctagaccca gctttcttgt acaaagtggt
      181 tcgatgggat cccgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      241 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      301 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      361 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      421 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      481 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      541 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      601 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      661 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      721 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      781 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
      841 catcgtggaa cagtacgaac gc
//
