LOCUS       sequence-55309-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Caspase-3 FRET biosensor, mammalian expression of 
            mAmetrine-DEVD-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: pmAmetrine-DEVD-tdTomato
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(92..113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      121 ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc accaacggca agctgaccct
      181 gaagttcatc tgcacctccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      241 gtcttacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctccttca aggacgacgg
      361 cagctacagg acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      481 catgaacgtg tgggacgcgt atatcacggc cgacaagcag aagaacggca tcaaagcgaa
      541 cttcaagatc gagcacaacg tcgaggacgg cggcgtgcag ctcgccgacg cgtaccagca
      601 gaacaccccc atcggcgacg gctccgtgct gctgcctgac aaccactacc tgagcttcca
      661 gagcaagctg ttcaaagacc ccaacgagca gcgcgatcac atggtcctgc tggagttcgt
      721 taccgccgcc gggatcactc tcggcggtac cggcagtggc agcggagatg aggtcgatgg
      781 tatggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
      841 gggctccatg aacgg
//
