LOCUS       sequence-55386-e        2687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-BBL1-ID43
FEATURES             Location/Qualifiers
     source          1..2687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..215
                     /gene="bla"
                     /label=AmpR promoter
     promoter        217..574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        217..408
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(240..260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      425..560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             609..1403
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             609..1403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(663..682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1273..1292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1591..1610)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1635..1682
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2011..2599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2500..2519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggataaccg tattaccgcc gaatgcaggt tgtcgcctgc agtccggatc ctggctgcag
       61 ccaggcacac gggctgcatt ccgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      181 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
      601 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
      661 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      721 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      781 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      841 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      901 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      961 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     1021 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     1081 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     1141 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1201 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1261 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     1321 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     1381 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     1441 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     1501 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1561 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     1621 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     1681 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     1741 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     1801 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     1861 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1921 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctg
//
