LOCUS       sequence-55392-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Zhao KW, Sikriwal D, Dong X, Guo P, Sun X, Dong JT
  TITLE     Oestrogen causes degradation of KLF5 by inducing the E3 ubiquitin 
            ligase EFP in ER-positive breast cancer cells.
  JOURNAL   Biochem J. 2011 Jul 15;437(2):323-33.
  PUBMED    21542805
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2011-07-15"; volume: "437(2)"; pages: "323-33"
COMMENT     Sequence Label: pcDNA3-FLAG-KLF5
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctgccac catggactac aaggacgacg atgacaaggg acctaagctt ggtaccgagc
      121 tcggatccat ggctacaagg gtgctgagca tgagcgcccg cctgggaccc gtgccccagc
      181 cgccggcgcc gcaggacgag ccggtgttcg cgcagctcaa gccggtgctg ggcgccgcga
      241 atccggcccg cgacgcggcg ctcttccccg gcgaggagct gaagcacgcg caccaccgcc
      301 cgcaggcgca gcccgcgccc gcgcaggccc cgcagccggc ccagccgccc gccaccggcc
      361 cgcggctgcc tccagaggac ctggtccaga caagatgtga aatggagaag tatctgacac
      421 ctcagcttcc tccagttcct ataattccag agcataaaaa gtatagacga gacagtgcct
      481 cagtcgtaga ccagttcttc actgacactg aagggttacc ttacagtatc aacatgaacg
      541 tcttcctccc tgacatcact cacctgagaa ctggcctcta caaatcccag agaccgtgcg
      601 taacacacat caagacagaa cctgttgcca ttttcagcca ccagagtgaa acgactgccc
      661 ctcctccggc cccgacccag gccctccctg agttcaccag tatattcagc tcacaccaga
      721 ccgcagctcc agaggtgaac aatattttca tcaaacaaga acttcctaca ccagatcttc
      781 atctttctgt ccctacccag cagggccacc tgtaccagct actgaataca ccggatctag
      841 atatgcccag ttctacaaat cagacagcag caatggacac tcttaatgtt tctatgtcag
      901 ctgccatggc aggccttaac acacacacct ctgctgttcc gca
//
