LOCUS       sequence-55406-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_wt_Venus_229
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..818)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
ORIGIN
        1 accatgccac cgctgccacc cttgtacagc tcgtccatgc cgagagtgat catggcggcc
       61 gctttagtag aaccttcacc agaaccaggt ttaccagaac cagaagttcc ggacttgtac
      121 agctcgtcca tgccgtcggt ggagttgcgg gccacggcgc tctcgtaaac ggtcaccttg
      181 ttgtagtcct tgtcgtggtt caggatctcg atgcggtggt ccacaaagtg atagtcgggc
      241 agcttcaccg ccttcttggc cctgtagatg gtcttgaagt caacgcggtg gtggccgccg
      301 ccctccagca gcagcttgtg cttgacgtcg cccttcagca cgccgtcgcg cacgtacatc
      361 ctctcggtgg aggcgtccca gccggtggtc ttcttctgca tcacggggcc gttggggggg
      421 aagttctcgc ccttgaggtg tatctcgtag atgaaggagt cctcctccat ggagatgtcg
      481 gacttcacct tcacgatgcc cttgtcctcg aaggtcatgg tgcgctccca agagtagccc
      541 tcggggaagg actgcttgaa gtagttgggg atgtcgtcgg ggtacttggt gaaggccctg
      601 ttgccgtagg cgaacgcggt ggtcagaatg tcgtaggaga agggcagggg ggctccctcc
      661 ttcacctcca ggttgatggt gttggtgccg tcgtagggct tgccctcgcc ctcgccctcg
      721 atcacgaagg cgtggccatt cacgttgccc tccatcttca gcttgatctt catgtcgggc
      781 ttgattacgc ccattgtggt ctcctcgccc ttgctcacgg atcccacaag cccttgctcc
      841 cta
//
