LOCUS       sequence-55413-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-natMX-tetO-Pcyc1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(35..222)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     143..167
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(246..317)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    join(537..555,556..578,579..597,598..620,621..639,640..662,
                     663..681,682..704,705..723,724..746,747..765,766..788,
                     789..807)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gatctatatt accctgttat ccctagcgga tctgccggta gaggtgtggt caataagagc
       61 gacctcatac tatacctgag aaagcaacct gacctacagg aaagagttac tcaagaataa
      121 gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta ttttttttat
      181 aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag aagtggcgcg
      241 ccctatttat catcatcgtc cttatagtcc ttatcgtcgt catctttgta atccttgtca
      301 tcgtcgtcct tgtagtccat attaattaac ctccaggatg ataaacggat cccccgaatt
      361 gatccggtaa tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat
      421 gctacaaagg acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt
      481 ttatatacat acagagcaca tgcatgccat agggtaccga gctcgacttt cacttttctc
      541 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      601 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      661 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      721 ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
      781 ttcacttttc tctatcactg atagggagtg gtaaactcga tc
//
