LOCUS       sequence-55414-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..223)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     144..168
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(247..318)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    join(538..556,557..579,580..598,599..621,622..640,641..663,
                     664..682,683..705,706..724,725..747,748..766,767..789,
                     790..808)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggatctatat taccctgtta tccctagcgg atctgccggt agaggtgtgg tcaataagag
       61 cgacctcata ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata
      121 agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt atttttttta
      181 taacttattt aataataaaa atcataaatc ataagaaatt cgcttattta gaagtggcgc
      241 gccctattta tcatcatcgt ccttatagtc cttatcgtcg tcatctttgt aatccttgtc
      301 atcgtcgtcc ttgtagtcca tattaattaa cctccaggat gataaacgga tcccccgaat
      361 tgatccggta atttagtgtg tgtatttgtg tttgcgtgtc tatagaagta tagtaattta
      421 tgctacaaag gacctaatgt ataaggaaag aatatttaga gaaaagaaga aaacaagagt
      481 tttatataca tacagagcac atgcatgcca tagggtaccg agctcgactt tcacttttct
      541 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
      601 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
      661 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      721 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      781 tttcactttt ctctatcact gatagggagt ggtaaactcg atcg
//
