LOCUS       sequence-55418-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L, Pines J, Nilsson
            J
  TITLE     Mechanisms controlling the temporal degradation of Nek2A and Kif18A 
            by the APC/C-Cdc20 complex.
  JOURNAL   EMBO J. 2013 Jan 23;32(2):303-14. doi: 10.1038/emboj.2012.335. Epub 
            2013 Jan 4.
  PUBMED    23288039
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.335"; journalName: "EMBO J"; date: "2013-01-23- 
            23"; volume: "32"; issue: "2"; pages: "303-14"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Venus-Flag (865)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(92..139)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     271..290
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    423..647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     636..653
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(694..759)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(814..835)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttggccaa ggaagttcct atactttcta gagaatagga
      121 acttcggaat aggaacttct aggtacgtga accatcaccc taatcaagtt ttttggggtc
      181 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      241 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      301 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      361 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      421 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      481 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      541 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      601 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      661 gcgggtttaa acgggccctc tagactcgag ctacttgtca tcgtcatcct tgtagtcgat
      721 gtcatgatct ttataatcac cgtcatggtc tttgtagtca gcccgggatc ccttgtacag
      781 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      841 gcgcttctcg ttggggtctt tgctcag
//
