LOCUS       sequence-55421-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Di Fiore B, Pines J
  TITLE     How cyclin A destruction escapes the spindle assembly checkpoint.
  JOURNAL   J Cell Biol. 2010 Aug 23;190(4):501-9.
  PUBMED    20733051
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-08-23"; volume: "190(4)"; pages: "501-9"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    172..296
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(321..326,327..344,345..351)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(471..490)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacatca aacaagtttg
      181 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
      241 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
      301 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      361 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      421 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      481 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      541 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      601 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      661 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      721 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
      781 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
      841 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaa
//
