LOCUS       sequence-55422-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Di Fiore B, Pines J
  TITLE     How cyclin A destruction escapes the spindle assembly checkpoint.
  JOURNAL   J Cell Biol. 2010 Aug 23;190(4):501-9.
  PUBMED    20733051
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2010-08-23"; volume: "190(4)"; pages: "501-9"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(95..142)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     274..293
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    426..650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     639..656
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(697..762)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
       61 agacgtcgcg gtgagttcag gctttttggc caaggaagtt cctatacttt ctagagaata
      121 ggaacttcgg aataggaact tctaggtacg tgaaccatca ccctaatcaa gttttttggg
      181 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
      241 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
      301 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
      361 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc
      421 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
      481 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
      541 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
      601 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
      661 tcagcgggtt taaacgggcc ctctagactc gagctacttg tcatcgtcat ccttgtagtc
      721 gatgtcatga tctttataat caccgtcatg gtctttgtag tcagcccggg atcccttgta
      781 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
//
