LOCUS       sequence-55423-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L, Pines J, Nilsson
            J
  TITLE     Mechanisms controlling the temporal degradation of Nek2A and Kif18A 
            by the APC/C-Cdc20 complex.
  JOURNAL   EMBO J. 2013 Jan 23;32(2):303-14. doi: 10.1038/emboj.2012.335. Epub 
            2013 Jan 4.
  PUBMED    23288039
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.335"; journalName: "EMBO J"; date: "2013-01-23- 
            23"; volume: "32"; issue: "2"; pages: "303-14"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Venus-Flag (865)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..82)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(95..805,806..808,809..811))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(137..158)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     486..505
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     745..766
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctagactc gagctacttg tcatcgtcat ccttgtagtc gatgtcatga tctttataat
       61 caccgtcatg gtctttgtag tcagcccggg atcccttgta cagctcgtcc atgccgagag
      121 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      181 ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
      241 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
      301 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
      361 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      421 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      481 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      541 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      601 actgcaggcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      661 tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      721 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      781 cggtgaacag ctcctcgccc ttgctcacca tccaccacac tggactagtg gatccgagct
      841 cggtaccaag ctta
//
