LOCUS       sequence-55425-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(227..245,246..268,269..287,288..310,311..329,330..352,
                     353..371,372..394,395..413,414..436,437..455,456..478,
                     479..497)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggatccccgg gttaattaac ctccaggatg ataaacggat cccccgaatt gatccggtaa
       61 tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg
      121 acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat
      181 acagagcaca tgcatgccat agggtaccga gctcgacttt cacttttctc tatcactgat
      241 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
      301 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
      361 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      421 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
      481 tctatcactg atagggagtg gtaaactcga tcgaggaatt gatctatatt acccagatct
      541 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
      601 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
      661 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
      721 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca
      781 gacgcgttga attgtcccca cgccgcgc
//
