LOCUS       sequence-55428-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pFA6a-kanMX-tetO-Pcyc1-GST
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..687)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(54..76)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 atctggcgcg cctcaacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa
       61 cgtggcttgc cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc
      121 aatttgtggg atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag
      181 gcacattggg tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg
      241 ggttacatga tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag
      301 catttcaggt agcttgctaa gaaaatcaac tttgagagtt tcaaagtctt tactatatgc
      361 aattctcgaa acaccgtatc taatatccaa aaccgctcct tcaagcattg aaatctctgc
      421 acgctctttt ggacaaccac ccaacatgtt gtgcttgtca gctatataac gtatgatggc
      481 catagactgt gttaatttaa catcaccatc aatataataa ggaagattgg gaaactccaa
      541 acccaattca aactttttgt ttcgccattt atcaccttca tcgcgctcat acaaatgctc
      601 ttcatatttt tcttcaagat attccaaaag aagtcgagtg ggttgcacaa ggcccttaat
      661 tttccaataa cctagtatag gggacatatt aattaacctc caggatgata aacggatccc
      721 ccgaattgat ccggtaattt agtgtgtgta tttgtgtttg cgtgtctata gaagtatagt
      781 aatttatgct acaaaggacc taat
//
