LOCUS       sequence-55429-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pDM291-tetR-tup11-Delta-70
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..713
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(166..185)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
       61 gaccgatcca gcctccgcgg ccccgaattc atatgtctag attagataaa agtaaagtga
      121 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
      181 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
      241 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
      301 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
      361 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
      421 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
      481 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
      541 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
      601 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
      661 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtgga tctgttagta
      721 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      781 tgttctcacc taagcttaga acctttacca aaggtgatgc ggagagatgg gta
//
