LOCUS       sequence-55431-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tandem dimer of pea ascorbate peroxidase (W41F mutant). This 
            construct has been replaced by APEX2 (construct 49386). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 tdAPX W41F
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttgcg gccgccacca tgggcaagag ctatcccacc gttagtcccg
      121 attaccagaa ggccattgaa aaggctaaga ggaagctcag aggttttatc gctgagaaga
      181 aatgcgctcc tctaattctc cgtttggcat tccactctgc tggtactttt gattccaaga
      241 caaagactgg tggtcctttc ggaacaatta agcaccaagc tgagcttgct catggtgcta
      301 acaacggtct tgatatcgcg gttaggctgt tggagcctat taaggagcaa ttccctattg
      361 tgagctatgc tgatttctac cagttggctg gtgttgttgc tgttgagatt accggtggac
      421 ctgaagttcc tttccaccct ggtagggagg acaagcctga gccaccacct gagggtcgct
      481 tgcctgatgc cactaagggt tctgaccatt tgagggatgt gtttggaaag gctatggggc
      541 ttagtgatca ggacattgtt gctctatctg gtggtcacac cattggagct gcacacaagg
      601 agcgttctgg atttgaggga ccgtggactt ctaatcctct catttttgac aactcatatt
      661 tcactgagtt gttgactggt gagaaggatg gccttcttca gttgccaagt gataaggcac
      721 ttttgactga ctctgtattc cgccctcttg ttgagaaata tgctgcggat gaagatgttt
      781 tctttgctga ttatgctgaa gcacatctta agctctctga gctgggcttc gccgaggctg
      841 gctccacctc tggttctggc aaaccgggct ccggtgaagg ctctac
//
