LOCUS       sequence-55466-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T
  TITLE     Arl13b regulates ciliogenesis and the dynamic localization of Shh 
            signaling proteins.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.
  PUBMED    21976698
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2011-12"; volume: "22(23)"; pages: "4694-703. Epub 
            2011 Oct 5"
COMMENT     Sequence Label: pDEST40-Arl13b11
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    111..335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     324..341
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(364..381)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(391..432)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    458..482
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
       61 cgcgtgggga taccccctag agccccagct ggttctttcc gcctcagaag ccatagagcc
      121 caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
      181 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
      241 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
      301 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac
      361 tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg
      421 gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca agaaagctgg
      481 gtcggcgcgc ccaccctttg agatcgtgtc ctgagcatca ccgttaggtc tctgtcgcac
      541 agccagggga ggcagcggct tcccatagaa atcattgtga cgtgtttcac caagaggctc
      601 aagttttgga agtctagtga ctttgggggt tccccagcca acaggagggg gaggttgggg
      661 aggcgtggga ctctttggag tagactcgtc tgtattgact ggttctaccc gatgactcct
      721 tttcattctt agtttcttgg ttttcttctt actgttttcc ccggactctg accctcgctg
      781 gtcctgctca tcctcactgt ccagctgctg cgacagtgcc tccctgtaac tgtccaccac
      841 tcgctcgtca gggttttgga ggcagcaatc tgcctcactc tgaggcgctg cttgctctgg
      901 ctccacttta tgttctagga gaccaacatc actgtccttc tccacggtct gcttcttttt
      961 ctctttt
//
