LOCUS       sequence-55467-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T
  TITLE     Arl13b regulates ciliogenesis and the dynamic localization of Shh 
            signaling proteins.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.
  PUBMED    21976698
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2011-12"; volume: "22(23)"; pages: "4694-703. Epub 
            2011 Oct 5"
COMMENT     Sequence Label: pDEST53-Arl13b20
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..47
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             59..79
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(789..813)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(845..862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa gcaggctccg cggccgcctt
       61 gtttaacttt aagaaggagc ccttcaccat gcagaaagac acaactgaac agcgagctct
      121 tgaggaacaa gagaaacgtg agagggctga acgagtccgg aagttaaggg aagaaagaga
      181 acgagagcag acggaacttg atgggaccag tggtctggct gagattgact caggaccagt
      241 tcttgcgaat cctttccaac ccattgcagc tgtaatcatt gagaatgaaa agaagcagga
      301 aaaagagaaa aagaagcaga ccgtggagaa ggacagtgat gttggtctcc tagaacataa
      361 agtggagcca gagcaagcag cgcctcagag tgaggcagat tgctgcctcc aaaaccctga
      421 cgagcgagtg gtggacagtt acagggaggc actgtcgcag cagctggaca gtgaggatga
      481 gcaggaccag cgagggtcag agtccgggga aaacagtaag aagaaaacca agaaactaag
      541 aatgaaaagg agtcatcggg tagaaccagt caatacagac gagtctactc caaagagtcc
      601 cacgcctccc caacctcccc ctcctgttgg ctggggaacc cccaaagtca ctagacttcc
      661 aaaacttgag cctcttggtg aaacacgtca caatgatttc tatgggaagc cgctgcctcc
      721 cctggctgtg cgacagagac ctaacggtga tgctcaggac acgatctcat aaaagggtgg
      781 gcgcgccgac ccagctttct tgtacaaagt ggtgataatt aattaagata aacccgctga
      841 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      901 tccttgaccc tggaa
//
