LOCUS       sequence-55485-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Wang L, Jin Q, Lee JE, Su IH, Ge K
  TITLE     Histone H3K27 methyltransferase Ezh2 represses Wnt genes to 
            facilitate adipogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22.
  PUBMED    20368440
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Apr 20. 107(16):7317-22."
COMMENT     Sequence Label: MSCVhygro-F-Ezh2
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatcctg gccaccatgg actacaagga
       61 cgacgatgac aagatgggcc agactgggaa gaaatctgag aagggaccgg tttgttggcg
      121 gaagcgtgta aaatcagagt acatgagact gagacagctc aagaggttca gaagagctga
      181 tgaagtaaag actatgttta gttccaatcg tcagaaaatt ttggaaagaa ctgaaacctt
      241 aaaccaagag tggaagcagc ggaggataca gcctgtgcac atcatgactt ctgtgagctc
      301 attgcgcggg actagggagt gttcagtcac cagtgacttg gattttccag cacaagtcat
      361 cccgttaaag accctgaatg cagtcgcctc ggtgcctata atgtactctt ggtcgccctt
      421 acaacagaat tttatggtgg aagacgaaac tgtcttacat aacattcctt acatggggga
      481 tgaagttctg gatcaggacg gcactttcat tgaagaacta ataaaaaatt atgatggaaa
      541 agtgcatggt gacagagaat gtggatttat aaatgatgaa atttttgtgg agttggtaaa
      601 tgctcttggt caatataatg atgatgatga tgacgatgat ggagatgatc cagatgaaag
      661 agaagaaaaa cagaaagatc tagaggataa tcgagatgat aaagaaactt gcccacctcg
      721 gaaatttcct gctgataaaa tatttgaagc catttcctca atgtttccag ataagggcac
      781 cgcagaagaa ctgaaggaaa aatataaaga actcacggag cagcagctcc caggtgctct
      841 gcctcctgaa tgtactccaa acatcgatgg accaaatgcc aaatctgttc agagggagca
      901 aagcttgcat tcatttcata cgctcttctg tcgacgatgt ttt
//
