LOCUS       sequence-55488-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEXV3-Myc-H-RAS_V12
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          86..182
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             236..262
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(269..298,299..301,302..835)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
     polyA_signal    846..979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(870..889)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     923..942
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttcagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg
       61 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
      121 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
      181 aggcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattcggag cgatgtaccc
      241 atacgatgtt ccagattacg ctccaccaac ggaatataag cttgttgttg ttggcgccgt
      301 cggtgtgggc aagagtgcgc tgaccatcca gctgatccag aaccattttg tggacgaata
      361 cgaccccact atagaggatt cctaccggaa gcaggtggtc attgatgggg agacgtgcct
      421 gttggacatc ctggataccg ccggccagga ggagtacagc gccatgcggg accagtacat
      481 gcgcaccggg gagggcttcc tgtgtgtgtt tgccatcaac aacaccaagt cttttgagga
      541 catccaccag tacagggagc agatcaaacg ggtgaaggac tcggatgacg tgcccatggt
      601 gctggtgggg aacaagtgtg acctggctgc acgcactgtg gaatctcggc aggctcagga
      661 cctcgcccga agctacggca tcccctacat cgagacctcg gccaagaccc ggcagggagt
      721 ggaggatgcc ttctacacgt tggtgcgtga gatccggcag cacaagctgc ggaagctgaa
      781 ccctcctgat gagagtggcc ccggctgcat gagctgcaag tgtgtgctct cctgacgcga
      841 attccgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      901 agtgaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      961 aagctgcaat aaacaagtta acaacaacat tg
//
