LOCUS       sequence-55494-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 soybean APEX (IMS)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..786)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagtcattag gcatcagcaa acccaagctc
       61 ggaaagcttt tggtgagcct cagcgtaatc agcaaagaag gcatcttcgt ccgctgcata
      121 tttatcaacg agagggcgga atacagggtc agacaaaaga gccttgtcag aaggtagctg
      181 aaggagacct tccttctcac cactcaacaa ctccgtgaag tatgagttgt cgaaaataag
      241 aggattagag gtccagggac cctcaaatcc agaacgctcc ttgtgtgcag ctccaatagt
      301 gtgaccccca gatagagcaa cgatatcttg gtcagtaagc cccatagctt tgccaaacac
      361 atctctcaaa tggtcagaac ccttagtggc atcgggcaag cgaccctctg gtggtggctc
      421 aggcttgtcc tctcttccag ggtggaatgg aaccttaggt ccacccgtga cctcaacggc
      481 aacaacgcca gccaactggt agaaatcggc gtagctcaaa atagggaact ccgccttgag
      541 tggctccaaa agcctaacag cgatgtcaag accgttgtta gcgctgtgag ccagttcggc
      601 agggtgcttg atggttccga agggtccacc ggtcttcgtg cccttgtcaa aggttccagc
      661 agagtggaat gccaaacgga gcattagagg agcgcatctc ttctcagcga tgaagcctct
      721 gagcttcttc ttcgccttct caacggcgtc ctggtaatca gcactcacag ttgggtaaga
      781 ctttccggtg ctgtccaggc ccagcanggg gttggggatg ggcttgcctg gatccctgga
      841 ctgaatgggg acggcgcccc gcagcccgac caccagcttc gcgccgagag ccagccccag
      901 cccgagcccc aggccgccgg cccaa
//
