LOCUS       sequence-55496-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pDM291-tetR'-tup11-Delta-70
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..704
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(157..176)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
       61 agcctccgcg gccccgaatt catatgtcta gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agcttggtgt agagcagcct acactgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttaaaagggg
      301 aaagctggca agatttttta cgcaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcaatgg agcaaaagta cattcagata cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaac gcgttatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg atctgttagt atgcagccgt
      721 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac
      781 ctaagct
//
