LOCUS       sequence-55497-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pUG6SP-tTA
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..716
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(169..188)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cccgaattaa ttcatatgtc tagattagat aaaagtaaag
      121 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      181 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      241 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
      301 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      361 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      421 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      481 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      541 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      601 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
      661 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt
      721 acagccgcgc gcgtacgaaa aacaattacg ggtctaccat cgagggcctg ctcgatc
//
