LOCUS       sequence-55517-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of myc tagged human RICTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, 
            Erdjument-Bromage H, Tempst P, Sabatini DM
  TITLE     Rictor, a novel binding partner of mTOR, defines a 
            rapamycin-insensitive and raptor-independent pathway that regulates 
            the cytoskeleton.
  JOURNAL   Curr Biol 2004 Jul 27;14(14):1296-302.
  PUBMED    15268862
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol";
            date: "2004-07-27"; volume: "14(14)"; pages: "1296-302"
COMMENT     Sequence Label: myc-Rictor corrected
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        191..209
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     191..208
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     222..241
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      326..335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             335..364
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgctggtttg actccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      121 ggcccacccc cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca
      181 tacacatacg atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg
      241 tgtccactcc caggtccaac tgcacctcgg ttctatcgat tgaattcccg aaccgacagt
      301 cggtctcttc accaaggcca ttcgcgccac catggagcaa aagctcattt ctgaggaaga
      361 tctcaatggt ggtggtggtg ggtcgacgat ggcggcgatc ggtcgtggcc gctctctgaa
      421 gaacctccga gtacgagggc ggaatgacag cggcgaggag aacgtcccgc tggatctgac
      481 ccgagaacct tctgataact taagagagat tctccaaaat gtggccagat tgcagggagt
      541 atcaaatatg agaaagctag gccatctgaa taactttact aagcttcttt gtgatattgg
      601 ccacagtgaa gaaaaactgg gctttcacta tgaggatatc ataatttgtt tgcggttagc
      661 tttattaaat gaagcaaaag aagtgcgagc agcagggcta cgagcgcttc gatatctcat
      721 ccaagactcc agtattctcc agaaggtgct aaaattgaaa gtggactatt taatagctag
      781 gtgcattgac atacaacaga gcaacgaggt agagaggaca caagcacttc gattagtcag
      841 aaagatgatt actgtgaatg ctt
//
