LOCUS       sequence-55523-e        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT,
            Coyle AJ, Liao SM, Maniatis T
  TITLE     IKKepsilon and TBK1 are essential components of the IRF3 signaling 
            pathway.
  JOURNAL   Nat Immunol. 2003 May;4(5):491-6.
  PUBMED    12692549
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Immunol."; date: "2003-05"; volume: "4(5)"; pages: "491-6"
COMMENT     Sequence Label: pEF-Bos MAL Flag
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          152..355
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     312..332
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1107..1118
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1128..1151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1152..1169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
       61 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
      121 ccgggcgccg tccaggcacc tcgattagtt ctcgtgcttt tggagtacgt cgtctttagg
      181 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
      241 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
      301 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
      361 gtgaggaatt ctctagagat ccctcgacct cgagtatggc atcatcgacc tccctcccag
      421 ctcctggctc tcggcctaag aagcctctag gcaagatggc tgactggttc aggcagaccc
      481 tgctgaagaa gcccaagaag aggcccaact ccccagaaag cacctccagc gatgcttcac
      541 agcctacctc acaggacarc ccactacccc caagcctcag ctcagtcacg tctcccagcc
      601 tgccacccac acatgcgagt gacagtggca gtagtcgctg gagcaaagac tatgacgtct
      661 gcgtgtgcca cagtgaggaa gacctggtgg ccgcccagga cctggtctcc tacttggaag
      721 gcagcactgc cagcctgcgc tgcttcctgc aactccggga tgcaacccca ggcggcgcta
      781 tagtgtccga gctgtgccag gcactgagca gtagtcactg ccgggtgctg ctcatcacgc
      841 cgggcttcct tcaggacccc tggtgcaagt accagatgct gcaggccctg accgaggctc
      901 caggggccga gggctgcacc atccccctgc tgtcgggcct cagcagagct gcctacccac
      961 ctgagctccg attcatgtac tacgtcgatg gcaggggccc tgatggtggc tttcgtcaag
     1021 tcaaagaagc tgtcatgcgt tgtaagctac tacaggaggg agaaggggaa cgggattcag
     1081 ctacagtatc tgatctactt ggatccattg aaggaaggat cgtcaaggac tacaaggacg
     1141 acgatgacaa gcaccaccac caccaccact gagcggccgc gactctagag tgagggtccc
     1201 cacctgggac ccttgagagt atcaggtctc ccacgtggga gacaagaaat ccctgtttaa
     1261 tatttaaaca gcagtgttcc ccatctgggt ccttgcaccc ctcactctgg cctcagccga
     1321 ctgca
//
