LOCUS       sequence-55527-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_wt_Venus_wt
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(79..783)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
ORIGIN
        1 ctcgcccttg ctcaccatcg cggccgcttt agtagaacct tcaccagaac caggtttacc
       61 agaaccagaa gttccggact tgtacagctc gtccatgccg tcggtggagt tgcgggccac
      121 ggcgctctcg taaacggtca ccttgttgta gtccttgtcg tggttcagga tctcgatgcg
      181 gtggtccaca aagtgatagt cgggcagctt caccgccttc ttggccctgt agatggtctt
      241 gaagtcaacg cggtggtggc cgccgccctc cagcagcagc ttgtgcttga cgtcgccctt
      301 cagcacgccg tcgcgcacgt acatcctctc ggtggaggcg tcccagccgg tggtcttctt
      361 ctgcatcacg gggccgttgg gggggaagtt ctcgcccttg aggtgtatct cgtagatgaa
      421 ggagtcctcc tccatggaga tgtcggactt caccttcacg atgcccttgt cctcgaaggt
      481 catggtgcgc tcccaagagt agccctcggg gaaggactgc ttgaagtagt tggggatgtc
      541 gtcggggtac ttggtgaagg ccctgttgcc gtaggcgaac gcggtggtca gaatgtcgta
      601 ggagaagggc aggggggctc cctccttcac ctccaggttg atggtgttgg tgccgtcgta
      661 gggcttgccc tcgccctcgc cctcgatcac gaaggcgtgg ccattcacgt tgccctccat
      721 cttcagcttg atcttcatgt cgggcttgat tacgcccatt gtggtctcct cgcccttgct
      781 cacggatccc acaagccctt gctccctagt cgactgcaga atgcgcagct tcctggcagg
      841 ctccccctg
//
