LOCUS       sequence-55530-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Santamaria A, Nagel S, Sillje HH, Nigg EA
  TITLE     The spindle protein CHICA mediates localization of the chromokinesin
            Kid to the mitotic spindle.
  JOURNAL   Curr Biol. 2008 May 20;18(10):723-9.
  PUBMED    18485706
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2008-05-20"; volume: "18(10)"; pages: "723-9"
COMMENT     Sequence Label: pcDNA3 CHICA (Nigg HS342)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             129..158
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             162..191
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta ccatggagca gaagctgatc tccgaggagg
      121 acctgaacga gcagaagctg atctccgagg aggacctgaa cgagcagaag ctgatctccg
      181 aggaggacct gaacctggag gtgctgttcc agggacccgg ggtaccagga tccatggctc
      241 tgctgtccga gggcctggac gagctgcccg ccgcctgcct gtcgccgtgc gggccgccca
      301 acccgaccga gctgttcagc gagtcacggc gcctggctct ggaggagctg gtggcgggcg
      361 gccccgaagc cttcgcggcc ttcctgcgac gcgagcgcct ggctcgtttc ctgaaccccg
      421 atgaggtgca cgccattctg cgcgcggcgg agaggccggg agaggagggc gcggcggcgg
      481 cggcggcggc cgaggactcg ttcggctcct cgcacgactg ctcttcgggc acctacttcc
      541 ccgagcagtc ggacctggag ccaccgctgt tggagcttgg ctggcccgcc ttctaccagg
      601 gcgcctaccg cggcgccacg cgtgtcgaga cgcacttcca gccccgcggc gctggcgaag
      661 gtggccccta cggctgcaag gacgctctgc gccagcagct ccgctcggcg cgagaggtga
      721 ttgcagtggt catggacgtg ttcacagaca tcgacatctt cagagacctg caagaaatat
      781 gcaggaaaca gggagttgct gtgtatatcc ttctggacca ggctctcctc tctca
//
