LOCUS       sequence-55532-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Golsteyn RM, Schultz SJ, Bartek J, Ziemiecki A, Ried T, Nigg EA
  TITLE     Cell cycle analysis and chromosomal localization of human Plk1, a 
            putative homologue of the mitotic kinases Drosophila polo and 
            Saccharomyces cerevisiae Cdc5.
  JOURNAL   J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17.
  PUBMED    7962193
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "1994-06"; volume: "107 ( Pt 6)"; pages: "1509-17"
COMMENT     Sequence Label: pRcCMV myc-Plk1 wt (Nigg RG6)
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             125..154
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgct tacatttgct tctgacacaa ctgtgttcac tagcaacctc aaacagacac
      121 catggagcag aagctgatct ccgaggagga cctgaacatg aattcctgca gcccgggatc
      181 catgagtgct gcagtgactg cagggaagct ggcacgggca ccggccgacc ctgggaaagc
      241 cggggtcccc ggagttgcag ctcccggagc tccggcggcg gctccaccgg cgaaagagat
      301 cccggaggtc ctagtggacc cacgcagccg gcggcgctat gtgcggggcc gctttttggg
      361 caagggcggc tttgccaagt gcttcgagat ctcggacgcg gacaccaagg aggtgttcgc
      421 gggcaagatt gtgcctaagt ctctgctgct caagccgcac cagagggaga agatgtccat
      481 ggaaatatcc attcaccgca gcctcgccca ccagcacgtc gtaggattcc acggcttttt
      541 cgaggacaac gacttcgtgt tcgtggtgtt ggagctctgc cgccggaggt ctctcctgga
      601 gctgcacaag aggaggaaag ccctgactga gcctgaggcc cgatactacc tacggcaaat
      661 tgtgcttggc tgccagtacc tgcaccgaaa ccgagttatt catcgagacc tcaagctggg
      721 caaccttttc ctgaatgaag atctggaggt gaaaataggg gattttggac tggcaaccaa
      781 agtcgaatat gacggggaga ggaagaagac cctgtgtggg actcctaatt acatagctcc
      841 cgaggtgctg agcaagaa
//
