LOCUS       sequence-55537-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Caspase-3 FRET biosensor, mammalian expression of 
            mAmetrine-DEVD-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: pmAmetrine-DEVD-tdTomato
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(220..243)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(418..437)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tccttacttg tacagctcgt ccatgccgta caggaacagg tggtggcggc
      181 cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      241 gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg
      301 tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc
      361 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct
      421 tcttctgcat tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga
      481 tcagcgtgcc gtcctgcagg gaggagtcct gggtcacggt caccagaccg ccgtcctcga
      541 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg taatcgggga
      601 tgtcggcggg gtgcttcacg tacgccttgg agccgtacat gaactggggg gacaggatgt
      661 cccaggcgaa ggg
//
