LOCUS       sequence-55539-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hanisch A, Wehner A, Nigg EA, Sillje HH
  TITLE     Different Plk1 functions show distinct dependencies on Polo-Box 
            domain-mediated targeting.
  JOURNAL   Mol Biol Cell. 2006 Jan;17(1):448-59. Epub 2005 Nov 2.
  PUBMED    16267267
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-01"; volume: "17(1)"; pages: "448-59. Epub 2005 
            Nov 2"
COMMENT     Sequence Label: pcDNA3 PBD wt (Nigg AH12)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             129..158
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             162..191
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta ccatggagca gaagctgatc tccgaggagg
      121 acctgaacga gcagaagctg atctccgagg aggacctgaa cgagcagaag ctgatctccg
      181 aggaggacct gaacctggag gtgctgttcc agggacccgg ggtacctaag gatccagtgt
      241 ggtggaattc cagatcttcg attgctccca gcagcctgga ccccagcaac cggaagcccc
      301 tcacagtcct caataaaggc ttggagaacc ccctgcctga gcgtccccgg gaaaaagaag
      361 aaccagtggt tcgagagaca ggtgaggtgg tcgactgcca cctcagtgac atgctgcagc
      421 agctgcacag tgtcaatgcc tccaagccct cggagcgtgg gctggtcagg caagaggagg
      481 ctgaggatcc tgcctgcatc cccatcttct gggtcagcaa gtgggtggac tattcggaca
      541 agtacggcct tgggtatcag ctctgtgata acagcgtggg ggtgctcttc aatgactcaa
      601 cacgcctcat cctctacaat gatggtgaca gcctgcagta catagagcgt gacggcactg
      661 agtcctacct caccgtgagt tcccatccca actccttgat gaagaagatc accctcctta
      721 aatatttccg caattacatg agcgagcact tgctgaaggc aggtgccaac atcacgccgc
      781 gcgaaggtga tgagctcgcc cggctgccct acctacggac ctggttccgc acccgcagcg
      841 ccatcatcct gcacctcagc aacggcagcg tgcagatca
//
