LOCUS       sequence-55542-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Hanisch A, Wehner A, Nigg EA, Sillje HH
  TITLE     Different Plk1 functions show distinct dependencies on Polo-Box 
            domain-mediated targeting.
  JOURNAL   Mol Biol Cell. 2006 Jan;17(1):448-59. Epub 2005 Nov 2.
  PUBMED    16267267
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-01"; volume: "17(1)"; pages: "448-59. Epub 2005 
            Nov 2"
COMMENT     Sequence Label: pcDNA3 PBD HK-AA (Nigg AH13)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..124
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             128..157
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             161..190
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             194..217
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttac catggagcag aagctgatct ccgaggagga
      121 cctgaacgag cagaagctga tctccgagga ggacctgaac gagcagaagc tgatctccga
      181 ggaggacctg aacctggagg tgctgttcca gggacccggg gtacctaagg atccagtgtg
      241 gtggaattcc agatcttcga ttgctcccag cagcctggac cccagcaacc ggaagcccct
      301 cacagtcctc aataaaggct tggagaaccc cctgcctgag cgtccccggg aaaaagaaga
      361 accagtggtt cgagagacag gtgaggtggt cgactgccac ctcagtgaca tgctgcagca
      421 gctgcacagt gtcaatgcct ccaagccctc ggagcgtggg ctggtcaggc aagaggaggc
      481 tgaggatcct gcctgcatcc ccatcttctg ggtcagcaag tgggtggact attcggacaa
      541 gtacggcctt gggtatcagc tctgtgataa cagcgtgggg gtgctcttca atgactcaac
      601 acgcctcatc ctctacaatg atggtgacag cctgcagtac atagagcgtg acggcactga
      661 gtcctacctc accgtgagtt cccatcccaa ctccttgatg aagaagatca ccctccttaa
      721 atatttccgc aattacatga gcgagcactt gctgaaggca ggtgccaaca tcacgccgcg
      781 cgaaggtgat gagctcgccc ggctgcccta cctacggacc tggttccgca cc
//
