LOCUS       sequence-55543-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVPT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(62..561)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(110..129)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     503..526
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    600..717
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 accatggtgg cgaccggtag cgctaggacg cgtaagctta ggcctggatc ccgctagagg
       61 tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg
      121 tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc
      181 tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa
      241 gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca
      301 tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct
      361 gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac
      421 caaagaacgg agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc
      481 cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg
      541 ggaaaagcgc ctcccctacc cggtactcga ccatcgtcga ggctagtctc gtgatcgata
      601 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
      661 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaa
//
