LOCUS       sequence-55546-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmOrange
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             139..849
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYEXA
                     EGRHSTGGMDELYK"
     polyA_signal    900..1034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(924..943)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     978..997
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccactagt ggcgcgccat taattaaagg ccggccagcg
      121 atcgccggac atcgattgat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
      181 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      241 gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc
      301 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcatttcac ctacggctcc
      361 aaggcctacg tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag
      421 ggcttcaagt gggagcgcgt gatgaactac gaggacggcg gcgtggtgac cgtgacccag
      481 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      541 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      601 atgtaccccg aggacggtgc cctgaagggc aagatcaaga tgaggctgaa gctgaaggac
      661 ggcggccact acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      721 cccggcgcct acatcgtcga catcaagttg gacatcacct cccacaacga ggactacacc
      781 atcgtggaac agtacgaang cgccgagggc cgccactcca ccggcggcat ggacgagctg
      841 tacaagtaag aattcaaggc ctctcgagcc tctagaacta tagtgagtcg tattacgtag
      901 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1021 gcaataaaca agttaacaac
//
