LOCUS       sequence-55548-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Baculovirus transfer vector with C-terminal His tag and FLAG 
            tag, preceded by a TEV protease cleavage site. Includes sites for 
            LIC cloning, and SacB gene for negative selection .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pFB-CT10HF-LIC
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(202..306)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(332..786)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(469..490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     680..699
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctcatcatt ggaacgttct tcggggcgaa actctcaagg atcttaccgc tgttgagatc
       61 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      301 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
      361 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      421 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      481 aagagtccac tattaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
      541 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
      601 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
      661 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
      721 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
      781 gcgcgtccca ttcgccattc aggctgcaaa taagcgttga tattcagtca attacaaaca
      841 ttaataacga agagatgaca gaaaaatttt cattctgtga cagagaaaaa gtagccgaag
      901 atgacggttt gtcacatgga gttggcagga tgtttgatta aaaacataac aggagaaatg
      961 cc
//
