LOCUS       sequence-55556-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T
  TITLE     Translation elongation can control translation initiation on 
            eukaryotic mRNAs.
  JOURNAL   EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 
            2013 Dec 19.
  PUBMED    24357599
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201385651"; journalName: "EMBO J"; date: "2014-01-1- 
            1"; volume: "33"; issue: "1"; pages: "21-34"
COMMENT     Sequence Label: pTH646-2µ-RLuc
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..201)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     122..146
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(370..634)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(454..477)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ttcgaccggc atgccggtag aggtgtggtc aataagagcg acctcatgct atacctgaga
       61 aagcaacctg acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta
      121 agagtcactt taaaatttgt atacacttat tttttttata acttatttaa taataaaaat
      181 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac caacgatttg
      241 acccttttcc atcttttcgt aaatttctgg caaggtagac aagccgacaa ccttgattgg
      301 agacttgacc aaacctctgg cgaagaagtc caaagcttgc atgcctgcag gtcgactcta
      361 gaggatccgt cgaaactaag ttcttggtgt tttaaaacta aaaaaaagac taactataaa
      421 agtagaattt aagaagttta agaaatagat ttacagaatt acaatcaata cctaccgtct
      481 ttatatactt attagtcaag taggggaata atttcaggga actggtttca accttttttt
      541 tcagcttttt ccaaatcaga gagagcagaa ggtaatagaa ggtgtaagaa aatgagatag
      601 atacatgcgt gggtcaattg ccttgtgtca tcatttactc caggcaggtt gcatcactcc
      661 attgaggttg tgcccgtttt ttgcctgttt gtgcccctgt tctctgtagt tgcgctaaga
      721 gaatggacct atgaactgat ggttggtgaa gaaaacaata ttttggtgct gggattcttt
      781 ttttttctgg atgccagctt aaaaagcggg ctccattata tt
//
