LOCUS       sequence-55561-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmOrange
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             100..807
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    900..1034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(924..943)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     978..997
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccactagt catcgattga tggtgagcaa gggcgaggag
      121 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
      181 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag
      241 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      301 cctcatttca cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      361 ttcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaacta cgaggacggc
      421 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      481 aagctgcgcg gcaccaactt cccctccgac ggccccgtga tgcagaagaa gaccatgggc
      541 tgggaggcct cctccgagcg gatgtacccc gaggacggtg ccctgaaggg caagatcaag
      601 atgaggctga agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag
      661 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg acatcaagtt ggacatcacc
      721 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
      781 accggcggca tggacgagct gtacaaggaa ttcggagccg gcgcgccatt aattaaaggc
      841 cggccagcga tcgccaaggc ctctcgagcc tctagaacta tagtgagtcg tattacgtag
      901 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1021 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcagg
//
