LOCUS       sequence-55566-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Guarguaglini G, Duncan PI, Stierhof YD, Holmstrom T, Duensing S, 
            Nigg EA
  TITLE     The forkhead-associated domain protein Cep170 interacts with 
            Polo-like kinase 1 and serves as a marker for mature centrioles.
  JOURNAL   Mol Biol Cell. 2005 Mar;16(3):1095-107. Epub 2004 Dec 22.
  PUBMED    15616186
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-03"; volume: "16(3)"; pages: "1095-107. Epub 
            2004 Dec 22"
COMMENT     Sequence Label: pEGFP Cep170 (Nigg PID200)
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(45..64)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(46..64)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(109..312)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(138..158)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        313..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cttgctcacc atggtggcga ccggtagcgc tagagcttgg gtctccctat agtgagtcgt
       61 attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat
      121 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      181 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      241 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      301 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      361 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      421 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      481 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
      541 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
      601 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
      661 ttactattaa taactaatgc atggcggtaa tacggttatc cacagaatca ggggataacg
      721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaa
//
