LOCUS       sequence-55574-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Cheng KC, Inglese J
  TITLE     A coincidence reporter-gene system for high-throughput screening.
  JOURNAL   Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
  PUBMED    23018994
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2012-10"; volume: "9(10):937. doi"; pages: " 
            10.1038/nmeth.2170"
COMMENT     Sequence Label: pCI-6.20
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(76..272)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      90..225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    327..418
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(359..378)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    432..480
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(748..769)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      121 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg
      181 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      241 cgggactatg gttgctgact aattgagatg cagatcgcag atctcgagcc cgggctagca
      301 cgcgtaagag ctcggtacct atcgatagag aaatgttctg gcacctgcac ttgcactggg
      361 gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag cgtatgttag
      421 tactatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt
      481 gcggccgctc caagtacctc ccgtacctta atattactta cttatcatgg tagcttgggc
      541 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      601 ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      661 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      721 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      781 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtga
//
