LOCUS       sequence-55575-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Cheng KC, Inglese J
  TITLE     A coincidence reporter-gene system for high-throughput screening.
  JOURNAL   Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
  PUBMED    23018994
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2012-10"; volume: "9(10):937. doi"; pages: " 
            10.1038/nmeth.2170"
COMMENT     Sequence Label: pCI-6.20
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             96..152
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
       61 agggcggaaa gatcgccgtg gaattcggaa gcggagctac taacttcagc ctgctgaagc
      121 aggctggaga cgtggaggag aaccctggac ctatgacttc gaaagtttat gatccagaac
      181 aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc
      241 ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt
      301 tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat attgagccag
      361 tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg
      421 gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt
      481 taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca tttcattata
      541 gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga
      601 ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc aaatctgaag
      661 aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa
      721 tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag
      781 gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta aaa
//
