LOCUS       sequence-55577-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Auld DS, Thorne N, Maguire WF, Inglese J
  TITLE     Mechanism of PTC124 activity in cell-based luciferase assays of 
            nonsense codon suppression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 
            10.
  PUBMED    19208811
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-03-3"; volume: "106(9)"; pages: 
            "3585-90. Epub 2009 Feb 10"
COMMENT     Sequence Label: pFLucWT
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(171..189)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctcttaaggc tagagtactt aatacgactc actataggct agcccgggct cgagatctgc
       61 gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
      121 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
      181 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      241 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
      301 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      361 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
      421 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
      481 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
      541 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
      601 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
      661 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
      721 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
      781 tttttggcaa
//
