LOCUS       sequence-55578-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Auld DS, Thorne N, Maguire WF, Inglese J
  TITLE     Mechanism of PTC124 activity in cell-based luciferase assays of 
            nonsense codon suppression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 
            10.
  PUBMED    19208811
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-03-3"; volume: "106(9)"; pages: 
            "3585-90. Epub 2009 Feb 10"
COMMENT     Sequence Label: pFLuc190UGA
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(164..182)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggctagagta cttaatacga ctcactatag gctagcccgg gctcgagatc tgcgatctaa
       61 gtaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa
      121 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      181 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      241 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      301 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
      361 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
      421 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
      481 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
      541 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
      601 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
      661 ggacaagtga attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
      721 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
      781 caatcaaatc attccggata ctgcgatttt aagtgttgtt cca
//
