LOCUS       sequence-55580-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cheng KC, Inglese J
  TITLE     A coincidence reporter-gene system for high-throughput screening.
  JOURNAL   Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
  PUBMED    23018994
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2012-10"; volume: "9(10):937. doi"; pages: " 
            10.1038/nmeth.2170"
COMMENT     Sequence Label: pCI-6.22
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..150
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
       61 ggcggaaaga tcgccgtgga attcggaagc ggagctacta acttcagcct gctgaagcag
      121 gctggagacg tggaggagaa ccctggacct atgacttcga aagtttatga tccagaacaa
      181 aggaaacgga tgataactgg tccgcagtgg tgggccagat gtaaacaaat gaatgttctt
      241 gattcattta ttaattatta tgattcagaa aaacatgcag aaaatgctgt tattttttta
      301 catggtaacg cggcctcttc ttatttatgg cgacatgttg tgccacatat tgagccagta
      361 gcgcggtgta ttataccaga ccttattggt atgggcaaat caggcaaatc tggtaatggt
      421 tcttataggt tacttgatca ttacaaatat cttactgcat ggtttgaact tcttaattta
      481 ccaaagaaga tcatttttgt cggccatgat tggggtgctt gtttggcatt tcattatagc
      541 tatgagcatc aagataagat caaagcaata gttcacgctg aaagtgtagt agatgtgatt
      601 gaatcatggg atgaatggcc tgatattgaa gaagatattg cgttgatcaa atctgaagaa
      661 ggagaaaaaa tggttttgga gaataacttc ttcgtggaaa ccatgttgcc atcaaaaatc
      721 atgagaaagt tagaaccaga agaatttgca gcatatcttg aaccattcaa agagaaaggt
      781 gaagttcgtc gtccaacatt atcatggcct cgtgaaatcc cgttagtaaa
//
